| Product Name | 5(6) - FAM, SE5 - (and - 6) - Carboxyfluorescein, succinimidyl ester; 5(6) - FAM, NHS ester |
| Size | 25 mg |
| Catalog # | AS-81006 |
| US$ | $68 |
Although FITC reagents have been more often used to prepare fluoresceinated bioconjugates, the low stability of FITC bioconjugates makes some researchers use amine-reactive succinimidyl esters of carboxyfluorescein (commonly called FAM) in bioconjugations. FAM reagents give carboxamides that are more resistant to hydrolysis. We have shown that FAM reagents require less stringent reaction conditions and give better conjugation yields, and the resulted conjugates have superior stability. We noted that FITC labeled peptides tend to deteriorate more quickly than the corresponding FAM conjugates.
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| Detailed Information |  Datasheet Material Safety Data Sheets (MSDS) |
| Storage | -20°C desiccated and protected from light |
| References | 1. Hahn M, et al. Electrophoresis 22, 2691-700; (2001).2. Sanders SJ et al.Mol Cell Probes 14, 249-53;(2000).3. Brandis JW et al. Nucleic Acids Res 27, 1912-8; (1999). |
| Molecular Weight | 473.39 |
| Molecular Formula | C25H15NO9 |
| Spectral Properties | Abs/Em = 494/519 nm |
| Solvent System | DMF or DMSO |
| Product Citations | Williamson, Patrick. et al. PloS One. 2, 729 (2007). |
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